Perlakuan Jenis Minyak Berbeda Sebagai Bahan Enkapsulasi Hidrolisat Ikan Gabus (Channa striata)

Syukri Syukri, Gatot Siswo Hutomo, Samliok Ndobe

Abstract


Salah satu sumber albumin adalah ikan gabus (Channa striata). Albumin dapat mengalami kerusakan yang diakibatkan proses oksidasi selama penyimpanan. Enkapsulasi dapat menjadi salah satu teknologi yang dapat mempertahankan mutu albumin dari kerusakan oksidatif. Tujuan penelitian ini untuk mengetahui kualitas ekstrak hidrolisat ikan gabus (C. striata) yang dienkapsilasi dengan minyak berbeda (VCO, zaitun, kemiri, atau wijen) dengan lama penyimpanan 8 minggu. Penelitian terdiri atas beberapa tahap yang meliputi ekstraksi hidrolisat ikan gabus, proses enkapsulasi (maltodekstrin + minyak sesuai perlakuan), dan dilakukan perlakuan penyimpanan 0, 2, 4, 6, dan 8 minggu pada suhu kamar (± 25°C). Parameter yang diamati adalah kadar albumin, kapasitas antioksidan, asam lemak bebas, kadar air, dan kadar abu. Hasil pengujian memperlihatkan perlakuan jenis minyak bahan enkapsulasi berpengaruh terhadap kadar albumin, sedangkan waktu penyimpanan tidak berpengaruh terhadap perubahan kadar albumin. Selain itu, perlakuan perbedaan jenis minyak bahan enkapsulasi dan waktu penyimpanan memberikan pengaruh terhadap kapasitas antioksidan, asam lemak bebas, kadar air, dan kadar abu ekstrak hidrolisat ikan gabus. Dari hasil penelitian ini dapat diketahui bahwa minyak kemiri merupakan bahan enkapsulasi terbaik, diiikuti oleh minyak wijen, minyak zaitun, dan VCO. Ekstrak hidrolisat ikan gabus yang dienkapsulasi dengan minyak kemiri hanya mengalami penurunan 0,79% setelah disimpan selama 8 minggu, lebih rendah dari perlakuan dengan minyak wijen (1,02%), minyak zaitun (1,35%), dan VCO (1,72%).

 

Abstract

Snakehead fish (Channa striata) has been known as a source of albumin. Albumin is susceptible to oxidation, so that it can be degraded during storage. The encapsulation method is one of technologies to maintain the quality of albumin. This study aimed to determine the hydrolyzed property of snakehead fish (C. striata) encapsulated with olive oil, virgin coconut oil (VCO), candlenut oil, or sesame oil during storage. The stages of research included the manufacture of snakehead fish hydrolysate, encapsulation with a combination of a treatment oil and maltodextrin, and storage time (0, 2, 4, 6, and 8 weeks) at room temperature (± 25°C). The parameters observed were albumin content, antioxidant capacity, free fatty acid, water content, and ash content. The results showed that the type of oil as an encapsulating material affected albumin levels, while storage time did not affect changes in albumin levels. In addition, treating different types of encapsulated oil and storage time affects the antioxidant capacity, free fatty acid content, water content, and ash content of snakehead fish hydrolysate. From the present study, it can be concluded that candlenut oil was the best encapsulation material, followed by sesame oil, olive oil, and VCO. The hydrolyzed snakehead fish extract, encapsulated with candlenut oil, only decreased by 0.79% after being stored for eight weeks, lower than treatment with sesame oil (1.02%), olive oil (1.35%), and VCO (1.72%).

 


Keywords


albumin; hidrolisat; enkapsulasi; ikan gabus

References


Alberghina, D., Casella, S., Giannetto, C., Marafioti, S., & Piccione, G. (2013). Effect of storage time and temperature on the total protein concentration and electrophoretic fractions in equine serum. The Canadian Journal of Veterinary Research, 77(4), 293‒296.

Alfarisy, M. U. (2014). Pengaruh jenis kelamin dan ukuran terhadap kadar albumin pada ikan gabus (Channa striata). Skripsi pada Fakultas Matematika dan Ilmu Pengetahuan Alam Institut Teknologi Sepuluh Nopember, Surabaya. Retrieved from https://repository.its.ac.id/452/1/1508100705-Paper.pdf

Alviodinasyari, R., Pribadi, E. S. & Soejoedono, R. D. (2019). Kadar protein terlarut dalam albumin ikan gabus (Channa striata dan Channa micropeltes) asal bogor. Jurnal Veteriner, 20(3), 436‒444. doi:10.19087/jveteriner.2019.20.3.436

Anraku, M., Kabashima, M., Namura, H., Maruyama, T., Otagiri, M., Gebicki, J. M., Furutani, N., & Tomida, H. (2008). Antioxidant protection of human serum albumin by chitosan. International Journal of Biological Macromolecules, 43, 159–164. doi: 10.1016/j.ijbiomac.2008.04.006

Association of Official Analytical Chemists (AOAC). (2005). Offcial Methods of Analysis of AOAC International (18th ed). AOAC International.

Arpi, N. (2013). Profil medium chain fatty acids (MCFA) dan sifat kimia minyak kelapa (virgin coconut oil/VCO, minyak simplah, pliek u, klentik, dan kopra) dibandingkan dengan minyak sawit. SAGU, 12(2), 23‒31.

Asfar, M., Tawali, A. B., Pirman, & Mahendradatta, M. (2019). Ekstraksi albumin ikan gabus (Channa striata) pada titik isoeletriknya. Jurnal Agercolere, 1(1), 6‒12.

Ayton, J., Mailer, R. J. & Graham, K. (2012). The Effect of Storage Conditions on Extra Virgin Olive Oil Quality (p. 16-23). Canberra: Rural Industries Research and Development Corporation.

Bakry, A. M., Abbas, S. A. B., Majeed, H., Abouelwafa, M.Y., Mousa, A., & Liang, L. (2016). Microencapsulation of oils: A comprehensive review of benefits, techniques, and applications. Comprehensive Reviews in Food Science and Food Safety, 15(1), 143–182. doi:10.1111/1541-4337.12179

Basuny, A. M. (2019). Antioxidants in Olive Oil. In Muzzalupo, I. (Ed.) Technological Innovation in the Olive Oil Production Chain (pp. 1-15). London: IntechOpen. doi: 10.5772/intechopen.80993

Bopitiya, D. & Madhujith, T. (2013). Antioxidant activity and total phenolic content of sesame (Sesamum indicum L.) seed oil. Tropical Agricultural Research, 24(3), 296–302. doi: 10.4038/tar.v24i3.8015

Badan Standardisasi Nasional (BSN). (1998a). SNI No. 4474:1998 tentang Minyak Zaitun Sebagai Minyak Makanan. Jakarta: Badan Standardisasi Nasional.

Badan Standardisasi Nasional (BSN). (1998b). SNI No. 4468:1998 tentang Minyak Wijen Sebagai Minyak Makan. Jakarta: Badan Standardisasi Nasional.

Badan Standardisasi Nasional (BSN). (2008). SNI No. 7381:2008 tentang Minyak kelapa virgin (VCO). Jakarta: Badan Standardisasi Nasional.

Badan Standardisasi Nasional (BSN). (2014). SNI No. 8074:2014 tentang Ekstrak Albumin Ikan Gabus. Jakarta: Badan Standardisasi Nasional.

Castellanos, I. J., Carrasquillo, K. G., Lo! Pez, J. D. J., Alvarez, M. & Griebenow, K. 2001. Encapsulation of bovine serum albumin in poly(lactideco-glycolide) microspheres by the solid-in-oil-in-water technique. Journal of Pharmacy and Pharmacology, 53, 167–178.

Christiansen, C. & Skotland, T. (2010). Changes of protein solutions during storage: a study of albumin pharmaceutical preparations. Biotechnology and Applied Biochemistry, 55, 121–130. doi: 10.1042/BA20090239

de Castro, L. L., Martins, M. C. C., Garcez, A. M., Pacheco, J. F. R., Cunha, F. V. M., Neto, J. M. M., de Freitas, M. C. L. & de Melo Cunha, L. A. (2014). Hypoalbuminemia and oxidative stress in patients on renal hemodialysis program. Nutrición Hospitalaria, 30(4), 952‒959. doi:10.3305/nh.2014.30.4.7667

Delles, R. M. & Xiong, Y. L. (2014). The effect of protein oxidation on hydration and water-binding in pork packaged in an oxygen-enriched atmosphere. Meat Science, 97, 181‒188. doi:10.1016/j.meatsci.2014.01.022

Duralliu, A., Matejtschuk, P., Stickings, P., Hassall, L., Tierney, R. & Williams, D.R. (2020). The influence of moisture content and temperature on the long-term storage stability of freeze-dried high concentration immunoglobulin G (IgG). Pharmaceutics, 12, 303. doi:10.3390/pharmaceutics12040303

Erwiyani, A.R., Martodihardjo, S. & Lukitaningsih, E. (2016). Antioxidant activity of dried strawberry juices (Fragaria vesca L.) emulgel preparation using candlenut oil and it’s diffusion. Indonesian Journal of Pharmacy, 27(3), 145–151. doi: 10.14499/indonesianjpharm27iss3pp145

Faizah, N. I. & Haryanti, S. (2020). Pengaruh lama dan tempat penyimpanan yang berbeda terhadap kandungan gizi umbi jalar (Ipomoea batatas) var. Manohara. Jurnal Akademika Biologi, 9(2), 8‒14.

Fauziah, M. U., Supriadin, A. & Berghuis, N. T. (2017). Aktivitas antioksidan ekstrak metanol pada ekstrak virgin minyak zaitun kemasan. al-Kimiya, 4(2), 61‒69. doi: 10.15575/ak.v4i2.5086

Fauzy, A. (2018). Enkapsulasi protein bovine serum albumin (BSA) pada matriks silika gel dari tetraetilortosilikat (TEOS) dan tetrametilortosilikat (TMOS) dengan metode sol-gel. Tesis pada Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Gadjah Mada, Yogyakarta. Retrieved from http://etd.repository.ugm.ac.id/home/detail_pencarian/159663#filepdf

Giunchedi, P., Conti, B., Genta, I., Conte, U. & Puglisi, G. (2001). Emulsion spray-drying for the preparation of albumin-loaded PLGA microspheres. Drug Development and Industrial Pharmacy, 27(7), 745–750. doi:10.1081/ddc-100107331

Handajani, S., Manuhara, G. J., & Anandito, B. K. (2010). Pengaruh suhu ekstraksi terhadap karakteristik fisik, kimia dan sensoris minyak wijen (Sesamum Indicum L.). Agritech, 30(2), 116–122. doi:10.22146/agritech.9682

Hanjaya, C., Pranata, F. S., & Swasti, Y.R. (2020). Quality of virgin coconut oil with addition of peppermint oil. agriTECH, 40(3), 215‒222. doi:10.22146/agritech.43321

Harianti. (2011). Ikan gabus (Channa striata) dan berbagai manfaat albumin yang terkandung di dalamnya. Jurnal Balik Diwa, 2, 18–25.

Hasibuan, N.E., Tamrin, & Muis, Y. (2017). Mikroenkapsulasi minyak ikan pora-pora (Mystacoleucus padangensis) menggunakan metode spray drying untuk aplikasi nutrisi makanan. Jurnal Kimia Mulawarman, 14, 108–114.

Irawan, C., Awalia, T. N., & Uthami, S. (2013). Pengurangan kadar asam lemak bebas (free fatty acid) dan warna dari minyak goreng bekas dengan proses adsorpsi menggunakan campuran serabut kelapa dan sekam padi. Konversi, 2(2), 29‒33.

Johnson, M. A., Fische, J. G. & Kays, S. E. (1992). Is copper an antioxidant nutrient? Critical Reviews in Food Science and Nutrition, 32(1), 1‒31. doi:10.1080/10408399209527583

Kabaran, S. (2019). Olive Oil: Antioxidant Compounds and Their Potential Effects over Health. In Lagouri, V. (Ed.) Functional Food (pp. 1-19). London: IntechOpen. doi:10.5772/intechopen.80993

Kusumaningrum, G. A., Alamsjah, M. A. & Masithah, E. D. (2014). Uji kadar albumin dan pertumbuhan ikan gabus (Channa striata) dengan kadar protein pakan komersial yang berbeda. Jurnal Ilmiah Perikanan dan Kelautan, 6(1), 25‒29. doi:10.20473/jipk.v6i1.11377

Lozano-Castellon, J., de Alvarenga, J. F. R., Vallverdú-Queralt, A., & Lamuela-Raventos, R.M. (2022). Cooking with extra-virgin olive oil: A mixture of food components to prevent oxidation and degradation. Trends in Food Science & Technology, 123 (2022), 28–36. doi:10.1016/j.tifs.2022.02.022

Marina, A. M, Che Man, Y. B., Nazimah S. A. H., & Amin I. (2009). Chemical properties of virgin coconut oil. Journal of the American Oil Chemists’ Society, 86(4), 301‒307. doi:10.1007/s11746-009-1351-1

Marreiro, D.N., Cruz, K.J.C., Morais, J.B.S., Beserra, J.B., Severo, J.S. & de Oliveira, A.R.S. (2017). Zinc and oxidative stress: Current mechanisms. Antioxidants, 6, 24. doi:10.3390/antiox6020024

Mekky, R.H., Abdel-Sattar, E., Segura-Carretero, A., & del Mar Contreras, M. (2021). Metabolic profiling of the oil of sesame of the Egyptian cultivar ‘Giza 32’ employing LC-MS and tandem MS-Based untargeted method. Foods, 10, 298. doi:10.3390/foods10020298

Mohamed, H. M. A. & Awatif, I.I. (1998). The use of sesame oil unsaponifiable matter as a natural antioxidant. Food Chemistry, 62(3), 269‒276. doi:10.1016/S0308-8146(97)00193-3

Montesqrit & Ovianti, R. (2013). Pengaruh suhu dan lama

penyimpanan terhadap stabilitas minyak ikan dan mikrokapsul minyak ikan. Jurnal Peternakan Indonesia, 15(1), 62‒68.

Musafira, Dzulkifli, Fardinah & Nizar. (2020). Pengaruh kadar air dan kadar asam lemak bebas terhadap masa simpan minyak kelapa mandar. KOVALEN : Jurnal Riset Kimia, 6(3), 224‒229.

Mustafa, A., Widodo, M.A. & Kristianto, Y. (2012). Albumin and zinc content of snakehead fish (Channa striata) extract and its role in health. International Journal of Science and Technology, 1(2), 1‒8.

Mustrini, I., Mappiratu & Nurakhirawati. (2016). Pemanfaatan getah biduri dalam produksi albumin ikan gabus (Ophiocephalus striatus). Kovalen, 2(3), 24-32.

Nedovic, V., Kalusevic, A., Manojlovic, V., Levic, S., & Bugarski, B. (2011). An overview of encapsulation technologies for food applications. Procedia Food Science, 1, 1806–1815. doi:10.1016/j.profoo.2011.09.265

Nevin, K.G. & Rajamohan, T. (2009). Virgin coconut oil supplemented diet increases the antioxidant status in rats. Food Chemistry, 99(2006), 260–266. doi: 10.1016/j.foodchem.2005.06.056

Ngginak, J., Rupidara, A.D.N. & Daud, Y. (2019). Kandungan vitamin C dari ekstrak buah ara (Ficus carica L.) dan markisa hutan (Passiflora foetida L.). Jurnal Sains dan Edukasi Sains, 2(2), 54‒59. doi:10.24246/juses.v2i2p54-59

Nurhidayah, B., Soekendarsi, E. & Erviani, A.E. (2019). Kandungan kolagen sisik ikan bandeng Chanos-chanos dan sisik ikan nila Oreochromis niloticus. Bioma : Jurnal Biologi Makassar, 4(1), 39‒47. doi:10.20956/bioma.v4i1.6341

Nurulain, S., Aziz, N.A., Najib, M.S., Salim, M.R. & Manap, H. (2021). A review of free fatty acid determination methods for palm cooking oil. Journal of Physics: Conference Series, 1921(2021), 012055. doi:10.1088/1742-6596/1921/1/012055

Obianwuna, U.E., Oleforuh-Okoleh, V.U., Wang, J., Zhang, H.J., Qi, G.H., Qiu, K. & Wu, S.G. (2022). Potential implications of natural antioxidants of plant origin on oxidative stability of chicken albumen during storage: A review. Antioxidants, 11, 630. doi:10.3390/antiox11040630

Olisekodiaka, M.J., Onuegbu, A.J., Ebesunun, O.M., Agbedana, E.O. & Taylor, G.O. (2011). Effects of storage temperature, ph and time on urinary albumin level. African Journal of Biomedical Research, 14, 73‒75.

Pramusita, N. (2019). Lama Penyimpanan Terhadap Kadar Air, Kadar Abu, Dan Kadar Serat Kasar Marshmallow Semangka. Skripsi. Universitas Semarang. Retrieved from https://repository.usm.ac.id/detail-jurnalmahasiswa-313.html

Putri, J.C.S., Haryanti, S. & Izzati, M. (2017). Pengaruh lama penyimpanan terhadap perubahan morfologi dan kandungan gizi pada umbi talas bogor (Colocasia esculenta (L.) Schott). Jurnal Biologi, 6(1), 49‒58.

Rahardjo, S. (2004). Kerusakan Oksidatif pada Makanan. Pusat Studi Pangan dan Gizi. Universitas Gadjah Mada; Yogyakarta

Raheja, A., Agarwal, A., Muthuvijayan, V., Chandra, T.S. & Natarajan, T.S. (2013). Studies on encapsulation of BSA, lysozyme and insulin through coaxial electro-spinning. Journal of Biomaterials and Tissue Engineering, 3(6), 669‒672. doi:10.1166/jbt.2013.1121

Rahmawanty, D., Risa, A., Malikhatun, N., Prima, H.R., Nani, K. & Effionora, A. (2017). Nanoparticle preparation and characterization of Haruan fish (Channa Striata) exctract contains albumin from South Kalimantan with lonic gelation method. International Journal of Drug Delivery, 9(2), 47–51. doi:10.5138/09750215.2070

Ramezanzade, L., Hosseini, S.F., Akbari-Adergani, B. & Yaghmur, A. (2021). Cross-linked chitosancoated liposomes for encapsulation of fish-derived peptide. LWT, 150, 112057. doi:10.1016/j.lwt.2021.112057

Rawendra, R.D.S., Kosasih, H. & Lo, D. (2019). Retention of albumin in Indonesian shortfin eel meat (Anguilla bicolor) by freeze-drying encapsulation using maltodextrin and gum arabic as coating materials. Journal of Physics: Conference Series, 1363(1), 12009. doi:10.1088/1742-6596/1363/1/012009

Romadhoni, A. R., Afrianto, E., Pratama, R. I., & Grandiosa, R. (2016). Extraction of snakehead fish [Ophiocephalus Striatus (Bloch, 1793)] into fish protein concentrate as albumin source using various solvent. Aquatic Procedia, 7, 4–11. https://doi.org/10.1016/j.aqpro.2016.07.001.

Sáez, M.I., Barros, A.M., Vizcaíno, A.J., López, G., Alarcón, F.J. & Martínez, T.F. (2015). Effect of alginate and chitosan encapsulation on the fate of BSA protein delivered orally to gilthead sea bream (Sparus aurata). Animal Feed Science and Technology, 210, 114–124. doi:10.1016/j.anifeedsci.2015.09.008

Shaah, M.A., Allafi, F., Hossain, Md.S., Alsaedi, A., Ismail, N., Ab Kadir, M.O. & Ahmad, M.I. (2020). Candlenut oil: review on oil properties and future liquid biofuel prospects. International Journal of Energy Research, 2021, 1‒23. doi:10.1002/er.6446

Siddique, B.M., Ahmad, A., Alkarkhi, A.F.M., Ibrahim, M.H. & Omar, M. (2011). Chemical composition and antioxidant properties of candlenut oil extracted by supercritical CO2. Journal of Food Science, 76(4), C535‒C542. doi:10.1111/j.1750-3841.2011.02146.x

Suryani, S., Sariani, S., Earnestly, F., Marganof, M., Rahmawati, R., Sevindrajuta, S., Mahlia, T.M.I. & Fudholi, A. (2020). A comparative study of virgin coconut oil, coconut oil and palm oil in terms of their active ingredients. Processes, 8, 402. doi:10.3390/pr8040402

Supriyadi & Rujita, A.S. (2013). Karakteristik mikrokapsul minyak atsiri lengkuas dengan maltodekstrin sebagai enkapsulan. Jurnal Teknologi dan Industri Pangan, 24(2), 201–208. doi:10.6066/jtip.2013.24.2.201

Thakur, V., Paroha, S. & Mishra, R.P. (2018). Free fatty acid profile of seven sesame (Sesamum indicum L.) verities. International Journal of Current Microbiology and Applied Sciences, 7(7), 3439‒3453. doi:10.20546/ijcmas.2018.707.399

Ulfindrayani, I.F. & A’yuni, Q. (2018). Penentuan kadar asam lemak bebas dan kadar air pada minyak goreng yang digunakan oleh pedagang gorengan di Jalan Manyar Sabrangan, Mulyorejo, Surabaya. Journal of Pharmacy and Science, 3(2), 17‒22. doi:10.53342/pharmasci.v3i2.111

Wan, Y., Li, H., Fu, G., Chen, X., Chen, F. & Xie, M. (2015). The relationship of antioxidant components and antioxidant activity of sesame seed oil. Journal of the Science of Food and Agriculture, 95, 2571–2578. doi:10.1002/jsfa.7035

Wiyani, L., Rahmawati, Aminah, Aladin, A., Mustafiah & Juniar, M.E. (2020). Antioxidant activity of Virgin Coconut Oil and Virgin coconut Oil Emulsion. Systematic Reviews in Pharmacy, 11(12), 973‒976.

Yusri, S., Tiffany & Sutanto, H. (2020). Candlenut oil encapsulation with Hidroxypropyl Methylcellulose (HPMC) for body lotion application. IOP Conference Series: Materials Science and Engineering, 1011, 012046. doi:10.1088/1757-899X/1011/1/012046

Zavareze, E.dR., Telles, A.C., El Halal, S.M. L., da Rocha, M., Colussi, R., de Assis, L.M, de Castro, L.A.S., Dias, A.R.G. & Prentice-Hernández, C. (2014). Production and characterization of encapsulated antioxidative protein hydrolysates from whitemouth croaker (Micropogonias furnieri) muscle and byproduct. LWT - Food Science and Technology, 59(2 Part 1), 841–848. doi:10.1016/j.lwt.2014.05.013

Zhao, M. & Zacharia, N.S. (2018). Protein encapsulation via polyelectrolyte complex coacervation: Protection against protein denaturation. The Journal of Chemical Physics, 149(16), 163326. doi:10.1063/1.5040346




DOI: http://dx.doi.org/10.15578/jpbkp.v17i2.845

Article Metrics

Abstract view : 332 times
PDF (Bahasa Indonesia) - 159 times

Refbacks

  • There are currently no refbacks.


JPBKP adalah Jurnal Ilmiah yang terindeks :


Creative Commons License

ISSN : 1907-9133(print), ISSN : 2406-9264(online)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.