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Optimum Age of Starter for Microbial Transglutaminase (MTGase) Production Produced by Streptomyces thioluteus TTA 02 SDS 14 and Characterization of Crude Enzyme


 
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1. Title Title of document Optimum Age of Starter for Microbial Transglutaminase (MTGase) Production Produced by Streptomyces thioluteus TTA 02 SDS 14 and Characterization of Crude Enzyme
 
2. Creator Author's name, affiliation, country Dewi Seswita Zilda; Research Center For Marine and Fisheries Product Processing and Biotechnology, Indonesia
 
2. Creator Author's name, affiliation, country Yusro Nuri Fawzya; Research Center For Marine and Fisheries Product Processing and Biotechnology, Indonesia
 
2. Creator Author's name, affiliation, country Lia Siti Nur'amaliyah; IPB; Indonesia
 
2. Creator Author's name, affiliation, country Hana Nurullita Prestisia; IPB
 
2. Creator Author's name, affiliation, country Nisa Rachmania Mubarik; IPB
 
2. Creator Author's name, affiliation, country Puspita Lisdiyanti; LIPI
 
3. Subject Discipline(s)
 
3. Subject Keyword(s) transglutaminase, starter age, Streptomyces thioluteus
 
4. Description Abstract

The condition of starter plays important role in enzyme production. Optimum starter will lead to the optimum yield of enzyme production. The experiments which were aimed to obtain the optimum  age for starter of Streptomyces thioluteus TTA 02 SDS 14  from solid as well as liquid medium had been carried out which would be used for transglutaminase production. Medium used for culture maintenance, starter optimization and enzyme production was described by Bahrim. The result showed that S. thioluteus TTA 02 SDS 14 was ready to be used as starter after being cultured  on solid medium continued in liquid medium each  for 6 days. The enzyme production in a bioreactor using optimized starter produced enzyme with the highest activity after being fermented for 2 days with 150 rpm agitation. The crude enzyme active optimally at 45-50 oC, pH of 6 and  no effect of metal ion and inhibitor on enzyme activity.  
 
5. Publisher Organizing agency, location :Agency for Marine and Fisheries Research and Human Resources, Indonesia
 
6. Contributor Sponsor(s) Research Center for Marine and Fisheries Product Processing and Biotechnology
 
7. Date (YYYY-MM-DD) 2017-05-01
 
8. Type Status & genre Peer-reviewed Article
 
8. Type Type
 
9. Format File format PDF
 
10. Identifier Uniform Resource Identifier https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/squalen/article/view/283
 
10. Identifier Digital Object Identifier (DOI) https://doi.org/10.15578/squalen.283
 
11. Source Title; vol., no. (year) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology; Vol 12, No 1 (2017): May 2017
 
12. Language English=en en
 
13. Relation Supp. Files
 
14. Coverage Geo-spatial location, chronological period, research sample (gender, age, etc.)
 
15. Rights Copyright and permissions Copyright (c) 2017 Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology