Optimasi Pertumbuhan Bakteri Laut Salinicola peritrichatus LBF-1-0025 dalam Senyawa Alkana

Ratna Cempaka Lingga, Ahmad Thontowi, Elvi Yetti, Anto Budiharjo, Isworo Rukmi, Yopi Yopi

Abstract


Abstrak

Pentana, dekana, pentadekana, dan parafin merupakan jenis kelompok senyawa alkana yang tidak dapat larut dalam air dan sulit terdegradasi. Sifat ini yang menjadikan senyawa alkana dapat mencemari lingkungan. Penelitian ini bertujuan untuk mengetahui konsentrasi optimum senyawa alkana bagi pertumbuhan isolat LBF-1-0025 dan mengidentifikasinya secara molekuler berdasarkan gen 16S rDNA. Dari skrining awal diketahui bahwa isolat LBF-1-0025 memiliki potensi tinggi dalam mendegradasi senyawa pentana, dekana, pentadekana, dan parafin. Optimasi pertumbuhan isolat LBF-1-0025 pada beberapa senyawa alkana dilakukan dengan menumbuhkannya dalam medium Artificial Sea Water (ASW) yang mengandung beberapa konsentrasi senyawa pentana, dekana, pentadekana, dan paraffin. Isolat LBF-1-0025 mampu tumbuh secara optimal pada pentana 150 ppm, dekana 200 ppm, pentadekana 150 ppm, dan parafin 200 ppm setelah 3 hari inkubasi. Hasil analisis sekuen gen 16S rDNA, bakteri LBF-1-0025 memiliki kemiripan sebesar 97% dengan Salinicola peritrichatus DY22.


The Growth Optimization of Marine Bacteria  Salinicola  peritrichatus LBF-1-0025 in Alkanes

Abstract

Pentane, decane, pentadecane, and paraffin are kind of groups of alkane compounds that are insoluble in water and difficult to degrade. This characteristic cause alkane compounds to pollute the environment. The aims of this research were to determine the optimum concentration of alkane compounds for the growth of isolate LBF-1-0025 and molecular identification based on 16S rDNA gene. From initial screening test, it was known that isolate LBF-1-0025 had high potential in degrading pentane, decane, pentadecane and paraffin compounds. Growth optimization of isolate LBF-1-0025 on several alkane compounds were carried out by culturing it in ASW medium containing various concentration of alkane (pentane, decane, pentadecane, and paraffin). Isolate LBF-1-0025 had optimum growth in pentane 150 ppm, decane 200 ppm, pentadecane 150 ppm and paraffin 200 ppm after 3 days incubation. Result analysis of 16S rDNA gene showed that isolate LBF-1-0025 had a similarity of 97% with  Salinicola  peritrichatus DY22.


Keywords


alkana; bakteri laut; 16S rDNA

Full Text:

PDF


DOI: http://dx.doi.org/10.15578/jpbkp.v12i2.344

Article Metrics

Abstract view : 98 times
PDF - 90 times

Refbacks

  • There are currently no refbacks.


Creative Commons License

ISSN : 1907-9133(print), ISSN : 2406-9264(online)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.