Pengaruh Pemberian Ekstrak Etanol Keong Matah Merah (Cerithidea obstusa) Terhadap Kadar Enzim Transaminase Tikus Putih (Rattus novergicus)

Abstract

Keong matah merah (Cerithidea obtusa) terbukti memiliki potensi farmakologis sebagai obat. Penelitian ini bertujuan menganalisis kadar enzim transaminase, yaitu serum glutamic pyruvic transaminase (SGPT) dan serum glutamic oxaloacetic transaminase (SGOT) pada serum tikus putih (Rattus novergicus) jantan galur Sprague Dawley yang diberi ekstrak etanol daging keong matah merah (C. obtusa) selama 28 hari. Kadar SGOT dan SGPT yang mengalami peningkatan secara bersamaan dapat menjadi indikator kerusakan hati. Hasil penelitian menunjukkan daging mentah segar keong matah merah (C. obtusa) mempunyai kadar air, protein, lemak, dan abu berturut-turut sebesar 74,22%; 11,99%; 0,63%, dan 8,40%. Ekstrak etanol daging mentah segar keong matah merah memiliki rendemen sebesar 3,41%. Penelitian ini menggunakan 24 ekor tikus putih jantan, yang dibagi secara acak menjadi 4 kelompok dan mendapatkan perlakuan yang berbeda selama 28 hari. Kelompok 1 (kontrol) beri akuades 2 ml/hari, kelompok 2 beri metotreksat 0,125 mg/kg BB/dua hari , kelompok 3 dan 4 diberi ekstrak etanol daging keong matah merah berturut-turut dengan dosis 100 dan 200 mg/kg BB/hari. Pengambilan sampel serum darah dilakukan pada hari ke-29 secara intrakardiak, kemudian dilakukan analisis kadar SGOT dan SGPT. Hasil penelitian menunjukkan bahwa ekstrak etanol daging keong matah merah dengan dosis 100 dan 200 mg/kg BB/hari secara signifikan menurunkan kadar SGOT dan SGPT pada serum tikus putih jantan.
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ABSTRACT

The red eyed snail (Cerithidea obtusa) has been shown to have pharmacological potential as a drug. This study aimed to anal,yze the levels of transaminase enzymes, namely serum glutamic pyruvic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT) in the serum of male white rats (Rattus novergicus) Sprague Dawley strain given ethanol extract of red eye snail meat (C. obtusa) for 28 days. Levels of SGOT and SGPT which increase simultaneously can be an indicator of liver damage. The results showed that fresh raw meat of red eyed snail (Cerithidea obtusa) had water, protein, fat and ash content respectively of 74.22%; 11.99%; 0.63%, and 8.40%. Ethanol extract of fresh raw meat of red eyed snail has a yield of 3.41%. This study used 24 male white rats which were randomly grouped into 4 groups and received different treatments for 28 days. Group 1 (control) was given a 2 ml of distilled water/day, group 2 was treated with methotrexate 0.125 mg/kg BW/two days, while groups 3 and 4 were fed with ethanol extract of red eye snail meat of 100 and 200 mg/kg BW/day respectively. Blood serum samples were taken by intracardiac method on the 29th day and then analyzed for SGOT and SGPT levels. The results showed that the red eye snail meat ethanol extract with doses of 100 and 200 mg/kg BW/day was significantly able to reduce SGOT and SGPT levels in male white rats serum.